doi: 10.1016/j.ajog.2010.06.045.ĭominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, et al. Isolation and characterization of true mesenchymal stem cells derived from human term decidua capable of multilineage differentiation into all 3 embryonic layers. Macias MI, Grande J, Moreno A, Dominguez I, Bornstein R, Flores AI. Furthermore, pluripotency markers indicate in both species a subpopulation of MSCs with true 'stemness', which could explain their high proliferation capacity, though possessing differences between human and marmoset in Lin28A and Sox2 expression. Studied human and common marmoset samples share many similar features such as most MSC markers and reduced MHC class I expression in amnion cells vs.
Little is known about MSC characteristics from the preclinical animal model common marmoset vs. Bisulfite sequencing of the Oct-4 promoter region displayed fewer methylations of CpG islands in the marmoset vs.
marmoset, whereas the marmoset exhibited significantly higher Lin28A values. Furthermore, human MSCs demonstrated the highest Sox2 levels vs. All cultured MSCs showed pluripotency marker expression like Oct-4A at passage 3 significantly decreasing over time (passages 6-12) while Nanog expression was highest in human bone marrow MSCs. For MSC markers, CD73 and CD105 levels remain unchanged in amnion MSCs and slightly decline in bone marrow at late passages CD166 is significantly higher expressed in human MSCs, CD106 significantly lower vs. Interestingly, MHC class I expression is significantly reduced in amnion MSCs until passage 6 in human and marmoset, but not in bone marrow cells. MSCs could be cultured more than 100 days (26 passages), but metabolic activity was significantly enhanced in amnion vs. Human and non-human primate MSCs were characterized for expression of MSC markers and capability of differentiation into mesenchymal lineages. MSCs derived from placental amnion and bone marrow samples from human and common marmoset were characterized in parallel over 12 passages to monitor similarities and significant differences (p ≤ 0.05, Student's t-test) in MSC markers and major histocompatibility complex (MHC) class I expression by immunohistochemistry, flow cytometry, real-time PCR, metabolic activity test, with special focus on pluripotency associated genes. Furthermore, despite intensive usage as preclinical animal model, little is known about MSCs of the common marmoset monkey.
However variety of MSC sources and general heterogeneity lead to controversial data in functional characterization. Multipotent stromal cells (MSCs) are among the key candidates in regenerative medicine.
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